Electrochemical Study of Trametes Versicolor Laccase Compatibility to Different Polyphenolic Substrates
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Electrochemical Study of Trametes Versicolor Laccase Compatibility to Different Polyphenolic Substrates
The aim of this electrochemical study was to ascertain which position of hydroxy groups on a benzene ring provides electroactive products after enzymatic oxidation by laccase originating from the Trametes versicolor mushroom, exhibiting intense redox signals that are exploitable for their amperometric determination. The electrochemical properties of phenol together with all isomers of benzenedi...
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An efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the hig...
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The regulation of culture conditions, especially the optimization of substrate constituents, is crucial for laccase production by solid fermentation. To develop an inexpensive optimized substrate formulation to produce high-activity laccase, a uniform design formulation experiment was devised. The solid fermentation of Trametes versicolor was performed with natural aeration, natural substrate p...
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The expression of laccase in the white rot fungus Trametes versicolor is regulated at the level of gene transcription by copper and nitrogen. We used reverse transcription-PCR to demonstrate that as the concentration of copper or nitrogen in fungal cultures was increased, an increase in laccase activity, corresponding to increased laccase gene transcription levels, was observed. In addition, we...
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There is longstanding environmental concern regarding endocrine disrupting chemicals and wastewater treatment facilities’ inability to remove them. Estrogens in particular, including 17 β-estradiol, 17 α-ethynylestradiol, and bis-phenol A, have been reported to depress cortisol levels and cause development of intersexual gonads in fish, along with various additional disruptions to the endocrine...
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2017
ISSN: 2227-9040
DOI: 10.3390/chemosensors5010009